Figure 38-6: DNA sites for L5 integration and excision. The L5 attP and M. smegmatis attB site each contain a 43bp common core which in attP is flanked by arm-type integrase-binding sites (P1, P2, P3, P4 and P5); two additional sites (P6 and P7, not shown) to the right of P5 - as well as P3 - are dispensable for both integration and excision. The mIHF host factor does not bind specifically to att site DNA but occupies the indicated areas in the presence of integrase. Integration requires both integrase (Int) and mIHF); excision requires Xis in addition to Int and mIHF. Xis binds specifically to four closely spaced sites (X1-X4) adjacent to P2. (for better image quality, click here for tif image, which may be quite large; may also require screen refreshing plus special software to view; or view larger tif in separate window)

Figure 38-1 ( ab, ), Figure 38-2, Figure 38-3, Figure 38-4, Figure 38-5, Figure 38-6

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