Figure 23-3: The bacteriophage SPP1 mature chromosome. The two main bars denote the mature phage sequence (1 to ~45.9 kb). The upper line of the bar represents the H (heavy) strand and the lower line the L (light) strand. The representation of the 16 EcoRI DNA fragments, the dispensable, early and late gene regions, early and late promoters; oriL and oriR, are according to figure 23-2. Fragment EcoRI-13t has one end generated by EcoRI digestion and the other by cleavage at the pac site by the terminase enzyme. The pac region is located within EcoRI DNA fragment 1 (uncleaved: pacLCR, solid black box indicated by vertical arrow). The cleaved pac site (pacCR, solid black oval, indicated by a vertical arrow) defines the first nucleotide of the SPP1 sequence. To present the full EcoRI DNA fragment 1, the first 718 bp (defining the EcoRI-13t fragment) are shown again in the upper main bar after position 44007 (broken line). Therefore, gene 1 is also presented twice. Due to imprecision of the headful cut mechanism the end of the mature SPP1 chromosome (~45.9 kb) is not precisely defined. The numbered hatched boxes denote phage-encoded products that have been genetically or biochemically characterized, whereas the empty ones indicate those of unknown function (3). (for better image quality, click here for tif image, which may be quite large; may also require screen refreshing plus special software to view; or view larger tif in separate window)

Figure 23-1, Figure 23-2, Figure 23-3, Figure 23-4, Figure 23-5, Figure 23-6, Figure 23-7, Figure 23-8

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