Figure 15-7: Collection of pseudorevertants obtained after passaging Q_β mutated in its 3' TD through wt or HF deficient host. The center shows the wild type Q_β structure together with transcription efficiency in the presence (+) and absence (-) of HF. The remarkable feature is that almost all suppressor mutations occur near the center of the five-way junction and destabilize ld IX. Furthermore, whether passaged in wt or HF deficient hosts, all pseudorevertants show strongly decreased dependence on HF. Mutant G4129C is a mutant, not a revertant. E1.7, E2.9 and A2.2 were sequenced only from nt 2349 to the 3' end. The transcription values refer to templates that were wild type from nt 1-2349 and revertant from 2349 to the 3' end. E1.7 and E2.9 also contain the suppressor mutation (C2405U). This change is predicted to increase translation of the replicase gene as it turns an inhibotory G-C pair into a G•U pair. (for better image quality, click here for tif image, which may be quite large; may also require screen refreshing plus special software to view; or view larger tif in separate window)

Figure 15-1, Figure 15-2 ( a,  b ), Figure 15-3, Figure 15-4, Figure 15-5, Figure 15-6, Figure 15-7, Figure 15-8, Figure 15-9 ( a,  b ), Figure 15-10, Figure 15-11, Figure 15-12, Figure 15-13,

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