Figure 12-6: Outline of several filamentous phage display strategies. A) pVII and pIX display: interacting proteins fused to their N-termini (signal sequences upstream, wild type pVII and pIX provided); pVIII display: large proteins fused to the N-terminus of pVIII displayed at low copy only (wild type pVIII provided); pVI display: proteins fused to the C-terminus displayed, though inefficiently (wild type pVI provided); pIII display: short peptides at the N-terminus of pIII can be uniformly displayed without disrupting infectivity. B) pVIII display: short peptides at the N-terminus of pVIII can be uniformly displayed without disrupting virus assembly; pIII display: large proteins fused to the N-terminus of pIII (wild type pIII provided). C) Fos-Jun C-terminal display: a Fos-protein XN fusion associates with N-terminally displayed Jun-pIII in the periplasm, and the entire sandwich incorporates into virions that contain the XN DNA sequence; selectively infective phage (SIP): protein Xa fused to the N-terminus of the C-domain of pIII and protein Ya fused to the C-terminus of the N1-N2 domains of pIII; XaYa interaction makes particles infectious. (for better image quality, click here for tif image, which may be quite large; may also require screen refreshing plus special software to view; or view larger tif in separate window)

Figure 12-1, Figure 12-2, Figure 12-3, Figure 12-4, Figure 12-5, Figure 12-6

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